Hayes, Alec J.,Kudithipudi, Chengalrayan,van der Hoeven, Rutger S.
申请号：
ES12191891
公开号：
ES2645763T3
申请日:
2008.12.11
申请国别(地区):
ES
年份:
2017
代理人:
摘要：
A method for reducing Cd levels in a tobacco plant, which comprises genetically modifying the plant by reducing the gene expression of the Nt heavy metal ATPase (NtHMA), wherein the NtHMA gene in modified or non-modified form modified is selected from the group consisting of: a gene comprising or consisting of a sequence that has at least 70% sequence identity with sec. with no. of ident.:1, and encoding an NtHMA transporter that has ATPase activity type P1B； a gene that comprises or consists of a sequence that has at least 70% sequence identity with sec. with no. of ident .: 3； a gene that comprises or consists of a sequence that has at least 70% sequence identity with sec. with no. Ident .: 47； a gene comprising or consisting of a sequence encoding a polypeptide that has at least 70% sequence identity with sec. with no. from ident.:2, wherein the polypeptide is an NtHMA transporter that has ATPase activity type P1B； and a gene comprising or consisting of a sequence encoding a polypeptide that has at least 70% sequence identity with sec. with no. of ident .: 49, wherein the polypeptide is an NtHMA transporter having P1B type ATPase activity； wherein the reduction of NtHMA gene expression is achieved by modification through mutagenesis of the NtHMA gene or through inactivation of the NtHMA gene, and where inactivation is achieved by the introduction of transposons and / or sequence elements of insertion (IS) in the genome of the plant, by Addressing Local Injuries Induced in Genomes ("TILLING"), or by introducing specific ribozymes of the target.Un método para reducir los niveles de Cd en una planta de tabaco, que comprende modificar genéticamente la planta mediante la reducción de la expresión génica de la ATPasa de metales pesados de Nt (NtHMA), en donde el gen de NtHMA en forma modificada o no modificada se selecciona del grupo que consiste en: un gen que comprende o que consiste en una secuencia que tiene al menos 70 % de identidad de secuencia con la sec. con n
