Manuel V. Borca,Luis L. Rodriguez,Peter W. Krug,Vivian K. ODonnell
申请号：
US14309548
公开号：
US09474797B1
申请日:
2014.06.19
申请国别(地区):
US
年份:
2016
代理人:
摘要：
We have developed an ASFV Georgia strain adapted to grow in Vero cell line. The resulting virus, ASF-GVAV, efficiently grows in Vero cells although it still is able to significantly replicate in primary cell cultures of swine macrophages. ASF-GVAV virus was successfully used as parental virus to develop several recombinant ASF viruses. The development of an ASFV adapted to grow in an established cell line is a significant advance for research and development of vaccine candidate strains using genetic manipulation based in the process of homologous recombination. The GVAVS can be utilized as a basis for large scale production of ASF vaccines.
