A biomaterial printing apparatus includes a support base, a movement device, a printing device, a first optical detection device, and a second optical detection device. The carrier is configured for a culture container putting thereon. The movement device is connected to the support base. The printing device is connected to the movement device. The first optical detection device is configured to detect the position of the injection needle of the printing device. The second optical detection device is configured to detect the position of the culture container. According to the detection of the first optical detection device and the second optical detection device, the biological material printing device can accurately move the injection needle to the injection position relative to the culture container, thereby improving the accuracy of printing the biological material.一種生物材料列印設備,包括一承載座、一移動裝置、一列印裝置、一第一光學偵測裝置、以及一第二光學偵測裝置。承載座用以供一培養容器放置。移動裝置連接承載座。列印裝置連接移動裝置。第一光學偵測裝置用以偵測列印裝置之注射針的位置。第二光學偵測裝置用以偵測培養容器之位置。依據第一光學偵測裝置以及第二光學偵測裝置之偵測,生物材料列印設備可將注射針精確地移動至對應於培養容器之注射位置,進而提高生物材料列印之精準度。