The invention would provide a method of measuring the physiological parameters of brain interstitial fluid (ISF) and brain extracellular space (ECS). The method is available to obtain the extract physiological parameters of the substances distributing, diffusing and eliminating in the brain ECS. The details are as follows: The head of the object was settled in magnetic resonance imaging (MRI)system the MRI contrast agent was injected into brain ISF the signal intensity changed by the MRI contrast agents was detected on MR images the distribution of the contrast agents in the brain can be quantitatively analyzed by the signal intensity the concentration of contrast agents and its time-dependent change of the different brain regions can be acquired. The invention can be feasible to quantify the indexes of the brain ISF distribution, fluidity and dissemination in the cerebral ECS by the signal intensity on MR images. The indexes including the anatomical and physiological parameters of the brain ISF and cerebral ECS can also be extracted by the invention.
