EMPRESA BRASILEIRA DE PESQUISA AGROPECUÁRIA - EMBRAPA;FUNDAÇÃO UNIVERSIDADE DE BRASÍLIA
发明人:
LIMA PEPINO DE MACEDO, Leonardo,GROSSI DE SÁ, Maria Fátima,MATTAR DA SILVA, Maria Cristina,ALMEIDA GARCIA, Rayssa,GODINHO MENDES, Reneida Aparecida,SALIBA ALBUQUERQUE FREIRE, Érika Valéria
申请号：
WO2018BR50087
公开号：
WO2018184083(A1)
申请日:
2018.03.29
申请国别(地区):
世界知识产权组织国际局
年份:
2018
代理人:
摘要：
The present invention describes a method for constructing structured nucleic acid molecules aiming to increase stability and efficiency thereof in supressing the expression of target genes in various organisms. The nucleic acid molecules according to the invention comprise a first region having at least 19 contiguous nucleotides, a second region complementary to the first, and a third region encoding a ribozyme. Stability of the nucleic acid molecule can also be increased by including incompatibility lock regions, which allow the ends of the molecule to bind. The invention also provides plants incorporating the taught sequences, demonstrating effectiveness thereof in controlling various agricultural pest species and illustrating one of the various possibilities of use.
