The invention relates to a method for cell defrosting, comprising a step of defrosting a cell suspension consisting of a freezing medium and of cells, followed by a step of removing said freezing medium by filtration, said step of removing the freezing medium being carried out without the previous or simultaneous supply of medium. In particular, the filter is hydrophobic or, before addition of the cell suspension to be frozen, has been covered with a hydrophobic liquid forming a temporary barrier between the filter and the cell suspension. During defrosting, said hydrophobic liquid is removed by the application of a driving force which makes the liquid pass through the filter.
