WANG, MENG,YANG, ZIZHEN,RADA, CRISTINA,NEUBERGER, MICHAEL
申请号：
ES10723293
公开号：
ES2498765T3
申请日:
2010.04.05
申请国别(地区):
ES
年份:
2014
代理人:
摘要：
An isolated or purified nucleic acid molecule comprising a nucleotide sequence encoding a functional mutant activation-induced cytidine deaminase (AID) whose amino acid sequence differs from the amino acid sequence of a human AID protein (SEQ ID No. 1 or SEQ ID No. 2) in at least one amino acid substitution selected from: (a) in at least one amino acid substitution in a residue selected from the group consisting of residues 34, 82 and 156, (b) in at least one amino acid substitution in the residue and at least one amino acid substitution in residue 156, (c) in at least one amino acid substitution in residue 35 and at least one amino acid substitution in residue 145, (d) in at least one substitution of amino acids in residue 34 and at least one amino acid substitution in residue 160, (e) in at least one amino acid substitution in residue 43 and at least one amino acid substitution in e residue 120, (f) at least two amino acid substitutions in which at least one substitution is in residue 57 and at least one substitution is in residue 81 or 145, (g) in at least one amino acid substitution in the residue 156 and at least one amino acid substitution in residue 82, (h) in at least one amino acid substitution in residue 156 and at least one amino acid substitution in residue 34, (i) in at least one amino acid substitution in residue 156 and at least one amino acid substitution in residue 157, (j) in at least one amino acid substitution in a residue selected from the group consisting of residues 10, 82 and 156, (k) in at least one amino acid substitution in residue 115, and (l) in at least one amino acid substitution in residue 120, in which the functional mutant AID protein has at least a 10-fold improvement in activity compared to the human AID protein in a papillation test No bacterialUna molécula de ácido nucleico aislada o purificada que comprende una secuencia de nucleótidos que codifica una citidina desaminasa inducida por activación (AID) mutante funcional cuya sec
