A lipid nanoparticle for antisense oligonucleotides inhibiting bcl-2 and belonging to the technical field of biology. The lipid nanoparticle for antisense oligonucleotides inhibiting bcl-2 is prepared by coating an antisense oligonucleotide with a membrane material, and the nucleic acid sequence is 5′；-TCT CCC AGC GTG CGC CAT-3′； (SEQ ID NO: 1), or 5′； UCU CCC AGC GTG CGC CAU 3′； (SEQ ID NO: 2). And a method for preparing the same is provided. The nanoparticles have a fairly good inhibitory effect on the growth of tumor cells and specific target genes, and particularly have a fairly good inhibitory effect on KB cervical cancer cells.
