The present disclosure provides a kit including a sterile package for applying a predetermined amount of at least one target agent to a medical device, the kit comprising at least one target agent, a container, a medical device disposed in the container, wherein at least a portion of the medical device is prepared to receive the at least one target agent and a port in communication with the container for allowing sterile passage of the at least one target agent to the medical device. The present disclosure also provides a method of applying a predetermined amount of a target agent to a medical device comprising, providing a prepared medical device in a sterile package having a port for allowing sterile passage of at least one target agent to the device and introducing the target agent into the container through the port to interact with the prepared medical device.