SOE, JORN BORCH,MIKKELSEN, JORN DALGAARD,MIASNIKOV, ANDREI,POVELAINEN, MIRA,PITKANEN, VIRVE
申请号：
AU2010214721
公开号：
AU2010214721B2
申请日:
2010.08.30
申请国别(地区):
AU
年份:
2012
代理人:
摘要：
The invention also encompasses the use of a lipolytic enzyme obtainable from one of the following genera: Streptomyces, Corynebacterium and Thermobifida in various methods and uses, wherein said lipolytic enzyme is capable of hydrolising a glycolipid or a phospholipid or transferring an acyl group 5 from a glycolipid or phospholipid to a acyl acceptor. Preferably, the lipolytic enzyme for use in these methods and uses comprises an amino acid sequence as shown in any one of SEQ. ID>； No.s 4, 5, 7, 8, 12, 14 or 16 or an amino acid sequence having at least 70% identity therewith of comprises a nucleotide sequence shown as SEQ ID No. 3, 6, 9, 13, 15 or 17 or a nucleotide sequence which has at least 70% identity herewith. The present invention also relates to a lipolytic enzyme capable of 0 hydrolising at least a galactolipid or capable of transferring an acyl group from a galactolipid to one or more acyl acceptor substrates, wherein the enzyme is obtainable from Steptomyces species and includes a lipolytic enzyme comprising an amino acid sequence as shown in SEQ ID No. 4 or an amino acid sequence which has at least 60% identity thereto. The enzyme may be encoded by a nucleic acid selected from the group consisting of: a) a nucleic acid comprising a nucleotide sequence 5 shown in SEQ ID No. 3； b) a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3 by the degeneration of the genetic code； and c) a nucleic acid comprising a nucleotide sequence which has at least 70% identity with the nucleotide sequence shown in SEQ ID No. 3.
