VATINE, GAD,SANCES, SAM,SVENDSEN, CLIVE,SAREEN, DHRUV,KERL, ALEXIS J.
申请号：
CA3035058
公开号：
CA3035058A1
申请日:
2017.08.29
申请国别(地区):
CA
年份:
2018
代理人:
摘要：
Described here are systems and methods for deriving both spinal motor neurons and brain microvascular endothelial cells from induced pluripotent stem cells using distinct methods and combining them in a chip format. Neurons cultured alone in chip microvolume displayed increased calcium transient function and chip-specific gene expression. When seeded with endothelial cells, interaction further enhanced neural function, elicited vascular-neural interaction, niche gene expression with enhanced in vivo-like signatures arising from the chip co-cultures. Development of novel media formulations further allow for improved readout of differentiation process, by eliminating additives that otherwise confound differentiation processes and resulting phenotypes.
