Embodiments related to methods of use of an image analysis system for identifying residual cancer cells after surgery are disclosed. In some embodiments, a patient-specific threshold used to detect abnormal cells in a surgical site can be determined. A medical imaging device can be configured to produce a set of images of an anatomy of a patient. An image analysis system, comprising one or more processors, can be configured to receive the set of images, and analyze the set of images to determine a patient-specific threshold to use to detect abnormal tissue of the patient.
