This invention designates that the method of rising the transformation efficiency of the plant in comparison with known agurobakuteriumu method until recently, is offered as topic. This invention the plant organization which inserts the agurobakuteriumu germ, designates that the coexistence process which is cultured with the coexistence nutrient medium which includes 3,6 jikuroro o anishin acid is included as one of feature.本発明は、従来公知のアグロバクテリウム法に比べて、植物の形質転換効率を上昇させる方法を提供することを課題とする。本発明は、アグロバクテリウム菌を接種した植物組織を、3,6-ジクロロ-o-アニシン酸を含む共存培地で培養する共存工程を含むことを特徴の1つとする。
