Compositions comprising combinations of chondroitin, glucosamine, connective tissue growth factor (CTGF), transforming growth factor beta 1 (TGFβ1) and WISP-2 are disclosed. Methods, uses and systems relating to the reduction of pain in a patient that emanates from a body area, preferably spine or joint are described. Methods of treatment or prevention are described for a disease or condition selected from degenerative disc disease, disc injury, pain, arthritis, or suspected arthritis.