DR NAGARAJAN SELVAMURUGAN,MS SALETH LEENA REGI,DR MARIAPPANADAR VAIRAMANI
申请号：
IN201741017615
公开号：
IN201741017615A
申请日:
2017.05.19
申请国别(地区):
IN
年份:
2017
代理人:
摘要：
Abstract Title: A nano-drug delivery system containing Silibinin for Bone formation Silibinin is a plant derived flavonolignan known for its multiple biological properties, but its role in the promotion of bone formation is not yet well studied. Moreover, the delivery of Silibinin is hindered by its complex hydrophobic nature and limits its bioavailability. Hence, in this study, we fabricated a drug delivery system using chitosan nanoparticles (CN) loaded with Silibinin (S) at different concentrations (20 uM, 50 uM, and 100 uM). They were then incorporated into scaffolds containing Alginate and Gelatin (Alg/Gel) for the sustained and prolonged release of Silibinin. The Silibinin loaded chitosan nanoparticles (SCN) were prepared by ionic gelation technique, and the scaffolds (Alg/Gel- SCN) were synthesized by the conventional method of freeze drying. The scaffolds were subjected to physicochemical characterization and material characterization studies. The addition of SCN did not affect the porosity of the scaffolds, yet increased the protein adsorption, degradation rates, and biomineralization. These scaffolds were biocompatible to mouse mesenchymal stem cells. The Silibinin loaded with 50 uM concentration in the scaffolds promoted osteoblast differentiation which was determined at cellular and molecular levels. Recent studies indicated the role of microRNAs (miRNAs) in osteogenesis, and we found that the Silibinin released from scaffolds regulated miRNAs that controlling the bone morphogenetic pathway (BMP). Hence, our results indicate the potential nature of sustained and prolonged release of Silibinin in bone formation, and thus, these Alg/Gel-SCN scaffolds can be candidature for bone tissue engineering applications.
