The present invention provides a breeding method of matsutake, which includes: preparing a culture medium, wherein the culture medium is formed by mixing grain crops, nutrient, and water sterilizing the culture medium to form a sterilized culture medium inoculating matsutake mycelium under aseptic condition to the sterilized culture medium to form a culture medium containing the inoculated matsutake mycelium and cultivating the culture medium containing the inoculated matsutake mycelium to obtain the mature matsutake mycelium the raw material of the culture medium used in the method of the present invention is easy to obtain, and simply inoculating the matsutake mycelium to the sterilized culture medium and cultivating the same under the appropriate temperature and time can produce the matsutake mycelium in a short period of time.本發明提供一種松茸之培育方法,其包括:齊備一培養基,其中該培養基係由五穀類作物、營養劑及水混合所形成;將該培養基予以滅菌,形成一已滅菌之培養基;將松茸菌絲體於無菌狀態接種至該已滅菌之培養基,形成一含有接種松茸菌絲體之培養基;以及培養該含有接種松茸菌絲體之培養基,以獲得成熟松茸菌絲體;本發明之方法所使用之培養基之原料容易取得,且僅需將松茸之菌絲體接種至已滅菌之培養基,並培養於適當溫度及時間,即可於短時間生成松茸菌絲體。
