A manufacturing method of mineralized scaffold and a device therefor are disclosed. The disclosed mineralized scaffold manufacturing device includes a bioscaffold having a pre-determined shape, an imaginary baseline extending from one end of the bioscaffold to the other thereof, a chamber having a space accommodating the bioscaffold and formed a corresponding sub-chamber space at two sides of the bioscaffold along the baseline. The disclosed is characterized by having the baseline as an axis, and the radial inner diameter of each sub-chamber space is gradually increased or decreased in sequence.本發明所提供礦化支架之製造裝置乃係包含有一預定形狀之生物支架:一假想之基準線,係自該生物支架之一端延伸至另一端;一腔室部,係以空間容納該生物支架,並沿該基準線於該生物支架之兩側分別形成一對應之子室空間;而其特徵則係在於,以該基準線為軸,各該子室空間之徑向內徑係呈依序漸次增加或減少者。(10)‧‧‧礦化支架之製造裝置(20)‧‧‧主體(21)‧‧‧身部(22)‧‧‧端部(23)‧‧‧進流道(231)‧‧‧進流口(40)‧‧‧載件(41)‧‧‧載體(42)‧‧‧樞軸(43)‧‧‧感磁體(44)‧‧‧固定片(45)‧‧‧墊片(46)‧‧‧穿孔(50)‧‧‧生物支架