Cryopreservative compositions include a carboxylated-polyamino acid, and at least one of an organic amphoteric agent or a polysaccharide. Methods of cryopreservation are also provided.Linvention concerne des compositions de cryoconservation comprenant un acide polyamino-carboxylé, et au moins un agent amphotère organique ou un polysaccharide. Linvention concerne également des méthodes de cryoconservation.