The present invention discloses a radioisotope 18F substituted thiamine, synthesis method, and use thereof in small animal PET/CT imaging. The radioisotope 18F substituted thiamine has a structure ofThe synthesis method comprises radiochemical synthesis by using existing precursors. In the present invention, the hydroxyl in the hydroxyethyl on the thiazole cycle of thiamine is replaced by radioisotope 18F, to prepare a PET tracer. The radioisotope 18F substituted thiamine of the present invention successfully enters the brains of various strains of mice, and the uptake in the brains of thiamine-deficient mice is obviously greater than that in normal control. The present invention is useful in the preparation of a brain imaging tracer for clinical trials of Alzheimers disease and tumors.
