The present invention relates to a method for measuring a physiological parameter of a subject by means of an optical measurement device, said method comprising the steps of: - setting into place the optical measurement device facing a skin surface of the subject, so that the object focal spot of the optical is positioned at a predetermined skin depth, - receiving by the photosensitive receiver light rays from the first object focal spot, at the predetermined skin depth, - analyzing the light rays received by the photosensitive receiver, and comparing the results of the analysis with known data, so as to determine the physiological parameter of the subject.本發明有關一種用於藉由一光學測量裝置來測量一受試者的生理參數的方法,該方法包含下列步驟:- 設置該光學測量裝置面向該受試者的皮膚表面,使得該光學物鏡的物體焦點係定位在一預決定的皮膚深度,- 由感光接收器接收源自在預決定皮膚深度之該第一物體焦點的光射線,- 分析由該感光接收器接收到的光射線,並將該等分析結果與已知數據比較,以便測定該受試者的生理參數。