用於擴增腫瘤浸潤性淋巴細胞之工程化人造抗原呈現細胞农业专利-农业学术服务平台

发明人:: VEERAPATHRAN, ANAND,维尔拉帕斯朗　安南,維爾拉帕斯朗　安南,GOKULDASS, AISHWARYA,格库达斯　艾许瓦亚,格庫達斯　艾許瓦亞,RABINOVICH, BRIAN,拉比诺维　布莱恩,拉比諾維　布萊恩,LOTZE, MICHAEL T.,洛兹　麦可,洛茲　麥可,维尔拉帕斯朗安南,維爾拉帕斯朗安南,格库达斯艾许瓦亚,格庫達斯艾許瓦亞,拉比诺维布莱恩,拉比諾維布萊恩,洛兹麦可,洛茲麥可

摘要：: In some embodiments, compositions and methods relating to isolated artificial antigen presenting cells (aAPCs) are disclosed, including aAPCs comprising a myeloid cell transduced with one or more viral vectors, such as a MOLM-14 or a EM-3 myeloid cell, wherein the myeloid cell endogenously expresses HLA-A/B/C, ICOS-L, and CD58, and wherein the one or more viral vectors comprise a nucleic acid encoding CD86 and a nucleic acid encoding 4-1BBL and/or OX40L and transduce the myeloid cell to express CD86 and 4-1BBL and/or OX40L proteins. In some embodiments, methods of expanding tumor infiltrating lymphocytes (TILs) with aAPCs and methods of treating cancers using TILs after expansion with aAPCs are also disclosed.於一些實施態樣中，本發明揭示關於經分離之人造抗原呈現細胞(aAPC)之組成物和方法，該組成物和方法包括aAPC，該aAPC包含經一或多種病毒載體轉導之髓樣細胞，諸如MOLM-14或EM-3髓樣細胞，其中該髓樣細胞內源性表現HLA-A/B/C、ICOS-L和CD58，且其中該一或多種病毒載體包含編碼CD86之核酸和編碼4-1BBL及/或OX40L之核酸並轉導該髓樣細胞以表現CD86和4-1BBL及/或OX40L蛋白。於一些實施態樣中，本發明亦揭示以aAPC擴增腫瘤浸潤性淋巴細胞(TIL)之方法及使用經aAPC擴增後之TIL治療癌症的方法。