Lars Kobberoee Skov,Leonardo De Maria,Tomoko Matsui
申请号:
US14627584
公开号:
US09611469B2
申请日:
2015.02.20
申请国别(地区):
US
年份:
2017
代理人:
摘要:
The present invention relates to a method for producing phytase variants which has at least 74% identity to a phytase derived from Citrobacter braakii and comprises at least two additional disulfide bonds as compared to this phytase. These phytase variants have modified, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensitiliby, and/or an modified glycosylation pattern. The invention also relates to the variants produced, DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.