in this method of invention very large quantities in tones of culture media will be recycled Large amount of nutrients present in the waste media as per experimental average weight of E.coli is 2 Pico gram and plate approximately 1 gm. Of nutrition which cannot be used by organisms during incubation period remaining nutrient used by this technique nutritional value does not decreases because of killed population of microorganisms also converted into amino acids and increase the nutritional value, In this method of invention a special kind of instrumentation used for the recycling which handle multiple processes U.V. chamber effectively killed all organisms from media, crusher crush media into small pieces and boiler boil effectively with controlled temperature. In this method of invention ail nutrients which present in waste media recycled at maximum level which can be used directly as media without any other treatment for another processes there could be tones of media as waste from all microbiological field. In the globalised world recycling of everything is important with reference to environment,cost and economy. Recycled media would be available at an reasonable rate also without any compromising of nutrition value compared to others.
