Provided is a platelet-rich plasma separator which can carry out the separation of a platelet-rich plasma with a small number of gamma radiation sterilized instruments and a platelet-rich plasma separation method. The platelet-rich plasma separator comprises a first syringe and a second syringe. The first syringe comprises a first syringe cylinder provided with a first port on which a blood collection needle can be mounted, a first cap detachable from the first port, a first gasket for sealing the first syringe cylinder fluid-tightly, which first gasket is reciprocated within the first syringe cylinder, and a first plunger provided detachably on the first gasket. The second syringe comprises a first hollow needle, which can be passed through the first gasket, a second syringe cylinder having a second port on which the first hollow needle can be mounted, a second cap detachable from the second port, a second gasket for sealing the second syringe cylinder liquid-tightly, which second gasket is reciprocated within the second syringe cylinder, and a second plunger provided detachable on the second gasket.
