The invention discloses a method for virus clearance of a cell culture medium, comprising the steps of: i) providing a bulk medium portion, comprising amino acids and glucose, and a first additive portion, comprising vitamins in aqueous solution； ii) subjecting the bulk medium portion to a high temperature short time treatment (HTST)； iii) passing the first additive portion through a virus retentive filter or an ultrafilter； and iv) after steps ii) and iii), mixing the bulk medium portion with the first additive portion to obtain a cell culture medium.
