The invention provides a formulation for the prevention/curing/treatment of diastema in mammals and animals by administering an effective amount of a composition comprising of an extract from at least one plant selected from Jatropha spp., Azadirachta indica, Acacia nelotica, Achyranthus aspera, Commiphora myrrha and Pongamia pinnata and a combination thereof. The invention also provides a process for preparing the same.