PROBLEM TO BE SOLVED: To manufacture a pigment-containing artificial skin capable of retaining, over an extended period, melanocytes effective for explorations of treatments of pigment freckle disorder.SOLUTION: The provided method for manufacturing a pigment-containing artificial skin comprises the following steps 1 through 3: (1) a step 1 of preparing a dermic layer by mixing a carrier and dermal fibroblasts and then incubating the resulting mixture (2) a step 2 of disseminating and incubating melanocytes within the upper portion of the dermic layer prepared at the step 1 and (3): a step 3 of preparing an epidermal layer by disseminating and incubating epidermal keratinocytes within the upper portion of the dermic layer prevailing after the step 2.COPYRIGHT: (C)2014,JPO&INPIT【課題】本発明の課題は、色素斑疾患の治療を探索するのに有効なメラノサイトが長期間保持された色素含有人工皮膚を製造する事である。【解決手段】以下の工程1~3を含む、色素含有人工皮膚の製造方法;(1)担体と真皮線維芽細胞を混合して培養し、真皮層を作製する工程1、(2)上記工程1にて作製した真皮層の上部に、メラノサイトを播種して培養する工程2、(3)上記工程2の後に、真皮層の上部に表皮角化細胞を播種して培養し、表皮層を作製する工程3。【選択図】なし
