An eye drop alignment assembly includes a ring that has a bottle of eye drops being extendable therethrough. The ring has a plurality of engagements thereon and the engagements are spaced apart from each other and are positioned at strategic points around the ring. A plurality of legs is each movably coupled to a respective one of the engagements. A plurality of extensions is each pivotally coupled to a respective one of the legs. Each of the extensions is positionable at a selected angle with respect to the respective leg for aligning with a respective top and bottom eyelid. Additionally, each of the extensions spaces the ring away from the eyeball associated with a top and bottom eyelids. In this way the ring aligns the bottle of eye drops with the eyeball for successfully dispensing eye drops onto the eyeball.