A needle groove body (1), a puncture frame body and a puncture frame,wherein the needle groove body (1)is a hollow cylindrical structure,and comprises a first baffle (11), a first fixing plate (12), a limiting plate (13), and a second fixing plate (14) which are connected in sequence.The present invention has the following advantageous effects:the needle groove body (1) has multiple different specifications and can adapt to puncture needles of different specifications in puncture operations;the needle groove body (1) is easy and convenient to fix and replace, easy to operate, and capable of allowing a doctor to operate with one hand;a u-shaped needle groove (3) can be formed between the first baffle (11) of the needle groove body (1) and a second baffle (21) as well as an inclined mounting plate of the puncture frame body so as to accommodatefree movement of apunctureneedlein the u-shaped needle groove (3); and the u-shaped needle groove (3), which is semi-open, can implement a function of needle-frame separation(separation of the puncture needle from the puncture frame) in puncture operationsat any time.