A portable multispectral imaging device comprises a first image sensing module, including at least one lens module and an image sensor. The image sensor is configured to acquire a plurality of images of light radiation, passing through the lens module, and the images include at least one first visible light image and an invisible light image. The center of the lens module and the center of the image sensor are aligned on an optical axis. Moreover, a plurality of light sources including at least one invisible light source are arranged to surround the first image sensing module. A flat-panel display module configured to show an image including the acquired images and previously saved images.
