The present invention relates to a disinfectant composition comprising hydrogen peroxide and silver ions in association with at least one quaternary ammonium salt with biocidal action and compatible with silver ions. The composition can be used for disinfecting a room and any objects contained therein, in particular healthcare environments, such as, for example, laboratories, doctors' surgeries, hospital rooms, ambulance compartments and the like, where it is important to have a biocidal activity at low dosages proportional to the average contamination present.