THE BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM
发明人:
FROLOV ILYA V.,WEAVER SCOTT C.,FROLOVA ELENA
申请号：
NZ60379009
公开号：
NZ603790A
申请日:
2009.01.23
申请国别(地区):
NZ
年份:
2014
代理人:
摘要：
Disclosed is an attenuated, recombinant alphavirus comprising an alphavirus nucleic acid, having: (i) an inactivated subgenomic promoter； and (ii) an insertion of an internal ribosomal entry site (IRES) between the end of nonstructural protein 4 (nsP4) coding sequence and initiating AUG of a subgenomic RNA coding sequence of the alphavirus, wherein the alphavirus is attenuated, wherein the IRES is not an encephalomyelocarditis virus internal ribosomal entry site (EMCV IRES) but is an IRES that functions in mammalian cells and wherein the alphavirus is attenuated. Also disclosed is a live, attenuated recombinant alphavirus comprising； a) a capsid gene positioned downstream from envelope glycoprotein genes and upstream from the 3&rsquo； UTR of the subgenomic RNA of the alphavirus； and b) an IRES introduced between the end of the envelope glycoprotein genes and the positioned capsid gene, wherein the IRES is not an encephalomyelocarditis virus internal ribosomal entry site (EMCV IRES) but is an IRES that functions in mammalian cells and wherein the alphavirus is attenuated.
