According to the present invention, a process is designed for preparation of PGPR bioformulation having superior viability by using Pseudomonas fluorescens VP5 isolated from tea rhizosphere. The invention is primarily designed to develop a PGPR formulation process for biocontrol of red root rot disease. This strain is useful as plant growth promoting rhizobacterium (PGPR) and biocontrol agent which can be formulated to produce antibiotic compounds that is active against the phytopathogen Poria hypolateritia. The process involves culturing of Pseudomonas fluorescens in a growth medium amended with colloidal chitin. The resulting mixture is then mixed with talc based carrier. The carrier with immobilized bioagent along with combination of vermicompost and farmyard manure is selected as efficient bioformulation for controlling root pathogen.