Patent No. 600548 Disclosed is a process for producing a protein concentrate from a toasted oilseed meal comprising: i) mixing the toasted oilseed meal with a first blending solvent to form a mixture； ii) optionally treating the mixture with phytase at a temperature and a pH suitable for phytase activity； iii) optionally adjusting the pH of the mixture to solubilize proteins in the mixture； iv) subjecting the mixture to a g-force sufficient to separate the mixture to form a) a fiber fraction, and b) a protein fraction comprising (i) an insoluble protein fraction, and (ii) a soluble protein fraction； v) separating the fiber fraction from the protein fraction and mixing the fiber fraction with a second blending solvent to form a fiber mixture； vi) treating the fiber mixture with a protease at a temperature and a pH suitable for protease activity； vii) subjecting the fiber mixture to a g-force sufficient to separate the fiber mixture to form: a) a second fiber fraction, and b) a hydrolyzed protein fraction, comprising (i) an insoluble protein fraction comprising partially hydrolyzed and un- hydrolyzed protein, and (ii) a soluble hydrolyzed protein fraction； viii) adjusting the pH of the protein fraction from step iv(b) to a pH suitable to precipitate proteins； ix) separating the precipitated proteins from the protein fraction； x) optionally combining the precipitated proteins and the hydrolysed protein fraction to form the protein concentrate.
