This invention relates to a method for detection of the efficacy of these antibiotics ciprofloxacin and amoxyclav and in particular, this invention relates to a method for detection by polymerase chain reaction (PCR). This invention also relates to a kit with composition having polymerase chain reaction (PCR) which improves the sensitivity of bacterial detection in middle ear infections. Furthermore, this invention also relates to the methods and kits described herein provide rapid, accurate, safe and cost efficient procedures.