Disclosed herein are systems, devices and methods for processing tissue, such as autologous tissue. Implementations of a Stromal Vascular Fraction (SVF) system are described that can isolate and wash harvested cells contained within various tissues, such as isolate and wash stem cells from fat tissue. The SVF system can minimize the handling and transferring of tissue and fluids, including minimizing the number of human interventions and manipulations required throughout processing. The SVF system can ensure sterility of processed tissue and harvested cells, as well as significantly reduce cost and time associated with the processing.
