ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI;UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION
发明人:
Ran BROSH, SR.,Ihor R. LEMISCHKA,Ning ZHENG
申请号：
US16093436
公开号：
US20190316150A1
申请日:
2017.04.12
申请国别(地区):
US
年份:
2019
代理人:
摘要：
The present invention relates to a system for F-box hormone receptor regulated protein expression in mammalian cells. The system includes a silencing nucleic acid molecule comprising a first promoter and an shRNA operably linked to the first promoter, where the shRNA silences expression of a target protein. The system also includes an expression nucleic acid molecule comprising a second promoter, an F-box hormone receptor operably linked to the second promoter, and a nucleic acid molecule encoding a fusion protein comprising a degron fused to the target protein, where the nucleic acid molecule encoding the fusion protein is operably linked to the second promoter. Also disclosed are vectors comprising the system of the present application and methods of use thereof.
