Methods for conveying metribuzin resistance and/or tolerance into non-resistant soybean germplasm are provided. In some embodiments, the methods include introgressing metribuzin resistance into a non-resistant soybean using one or more nucleic acid markers for marker-assisted breeding among soybean lines to be used in a soybean breeding program, wherein the markers are linked to and/or associated with metribuzin resistance and/or tolerance. Also provided are single nucleotide polymorphisms (SNPs) associated with resistance or tolerance to metribuzin. Soybean plants and seeds produced by any of the disclosed methods are provided.
