A surface disinfecting assembly for sterilizing a sole of a shoe includes a box that is positionable on a floor and is positioned adjacent to a selected area such that the box can be stepped in. A pad is positionable in the box and the pad is infused with a fluid antiseptic. In this way the pad sanitize a sole of the shoe thereby inhibiting the shoe from transferring bacteria into the selected area. A cushion is positionable adjacent to the box and the cushion can be stepped upon. The cushion is comprised of a fluid absorbent material for absorbing the fluid antiseptic from the sole of the shoe after the pad is stepped upon.
