A dried vitrigel membrane is produced by a method including the following steps of (1) a step of keeping a hydrogel in the inside of a wall surface mold with a shape the same as the desired shape disposed on a substrate, and discharging a part of free water within the hydrogel from a gap between the substrate and the wall surface mold (2) a step of removing the wall surface mold from the top of the substrate (3) a step of drying the hydrogel to remove the residual free water, thereby fabricating a vitrified dried hydrogel (4) a step of rehydrating the dried hydrogel to fabricate a vitrigel membrane and (5) a step of redrying the vitrigel membrane to remove free water, thereby fabricating a vitrified dried vitrigel membrane.