A knot placement device (100) allows a physician to apply a knot for securing two or more suture ends extending from an incision in a vessel or organ of a patient relative to each other in order to seal an opening in the vessel or organ. The knot placement (100) device has a handle (102) and an elongate shaft (104) and a push rod (118) slidably inserted in said shaft (104). A knot is disposed in the distal end of the shaft (104). An actuator (106) on the handle may be depressed to distally advance said push rod (118) relative to said shaft (104) and thereby distally advance said knot. The knot may include a knot body (124) having an inner cavity and a plug (126) sized to fit securely within the inner cavity. In use, the plug (126) may be inserted into the inner cavity of the knot body (124) to fixedly hold two or more suture ends (34) between the knot body (124) and the plug (126).
