The present invention provides a method of preparing an injection solution. The method includes: (1) storing a mixture of a blood sample and a divalent cation chelating agent at a temperature between 2 and 12 degrees Celsius for a period of from 3 hours to 72 hours so as to have the mixture with two or more separate layers, wherein a liquid in one of the separate layers contains small somatic stem cells such as SB cells (2) collecting the liquid from the mixture and (3) after collecting the liquid from the mixture, mixing the liquid with a solution free from Ca2+ into the injection solution. The injection solution can be used for treating an autoimmune disease, such as ankylosing spondylitis, rheumatoid arthritis, or Grover’s disease.本發明提供一種製備注射液的方法,其係包括:(1)將血液樣本和二價陽離子螯合劑的混合液放在溫度為攝氏2至12度的環境中靜置3至72小時,使混合液分成兩層或兩層以上(亦即混合液形成兩個或兩個以上的分離層),其中一個分離層內的液體含有小體幹細胞,例如SB細胞;(2)從混合液收集或取得該液體;以及(3)在收集或取得該液體之後,將該液體和無鈣離子(Ca2+)的溶液進行混合,因繼而形成所述的注射液。此注射液可以用於治療自體免疫疾病,例如僵直性脊椎炎、類風濕性關節炎或葛瑞夫茲氏症。10‧‧‧步驟11‧‧‧步驟12‧‧‧步驟13‧‧‧步驟14‧‧‧步驟
