A method of from parental polypeptide preparation condition active peptides,It is the following steps are included: increase the net charge of parental polypeptide by using one of sum of codon of uncharged amino acid residue in sum and reduction DNA selected from the codon for increasing amino acid residue electrically charged in DNA or multiple technologies to develop the step of DNA of coding parental polypeptide is to generate mutant DNA； Mutant DNA is expressed to obtain mutant polypeptide； The alternative condition active peptides from the mutant polypeptide for showing activity reduction compared with the allovitalism in the second test under the second value in condition of the same race in the first test under a kind of the first value of condition. Additionally provide condition active peptides, pharmaceutical composition, nano particle and its drug conjugate including the condition active peptides and application thereof.一種從親本多肽製備條件活性多肽的方法，其包括以下步驟：通過使用選自增加DNA中帶電荷的氨基酸殘基的密碼子的總數和减少DNA中不帶電荷的氨基酸殘基的密碼子的總數中的一種或多種技術來增加親本多肽的淨電荷以演變編碼親本多肽的DNA從而産生突變DNA的步驟；表達突變DNA以獲得突變多肽；從在一種條件的第一值下的第一測試中與在同種條件的第二值下的第二測試中的同種活性相比表現出活性降低的突變多肽中選擇條件活性多肽。還提供了條件活性多肽、包括所述條件活性多肽的藥物組合物、納米顆粒和其藥物綴合物及其用途。
