The present invention provides a lozenge preparation comprising a probiotic organism such as lactobacillus in different concentrations and placed in a specific location in the lozenge where survivability is preserved for a long time. The lozenge preparation is not limited to the probiotic inclusion but also in association with a varied dosage of prebiotics, such as Inulin, an oligosaccharide. The preparation may include sugar or may be sugar free. The invention further provides methods for producing such lozenges and uses for modulating embodiment and forming of center-tilled lozenges.
