Methods for the treatment of cancer using double negative T cells (DNTs) anda PD-1 or PD-L1 inhibitor are described. Tumors treated with DNTs and a PD-1 inhibitor exhibited increased DNT cell infiltration and increasedcytotoxicitytowards non-small cell lung cancer (NSCLC) cells. Also described arecompositions and kits comprising DNTs and a PD-1 or PD-L1 inhibitor and theiruse for the treatment of cancer.