#$%^&*AU2017201762A120170406.pdf#####ABSTRACT Tissue patches and associated systems and methods are described. Tissue patches can be made by applying a compressive force to a liquid medium comprising fibrinogen (and/or fibrin) between two surfaces (e.g., within a syringe or other chamber). A filter can be placed within or near the volume in which the compressive force is applied to the liquid medium such that unwanted material (e.g., water, blood cells, and the like) is passed through the filter while desirable components (e.g., fibrin, fibrinogen, and/or other desirable components) are retained by the filter to form the patch. In this way, the concentration of fibrin (and/or fibrinogen) within the liquid medium can be increased, potentially dramatically, as the compressive force is applied to the liquid-containing composition. In some embodiments, at least a portion of the fibrinogen and/or fibrin can chemically react (e.g., the fibrinogen can polymerize to form fibrin and/or the fibrin can cross-link) during application of the compressive force. Reaction and concentration can lead to the formation of a highly-concentrated, mechanically robust patch that can be handled relatively easily and provide good structural reinforcement at a wet site, such as a bleeding wound. In some embodiments, a kit is provided, comprising an unsupported solid matrix comprising fibrin at a concentration of at least about 10 grams per litre of the solid matrix, wherein the solid matrix is sterile and configured for application to a tissue surface, and the solid matrix has a tensile strength of between about 175 kPa and about 650 kPa when measured as a true stress at break； and a primer composition comprising a wateractivated polymeric adhesive.
