The present invention relates to a kit for influenza vaccine, comprising: a first vaccine composition including a Flu A of H1N1, a Flu A of H3N2, and a first Flu B and a second vaccine composition including a second Flu B different from the first Flu B. A use method of the kit is also provided herein, wherein the kit is administrated to an individual. The method comprises the following steps: a. administrating a first vaccine composition to the individual and b. administrating a second vaccine composition to the individual after 1 to 10 days of the administration of the first vaccine composition. Thereby, the prevention from Influenza will be more effective and broader.本發明涉及一種流感疫苗套組,其包括:一第一疫苗組合物,其包括:一H1N1類之A型流感病毒、一H3N2類之A型流感病毒以及一第一B型流感病毒;以及一第二疫苗組合物,其包括:與該第一B型流感病毒不同之一第二B型流感病毒。本發明亦提供一種上述之流感疫苗套組的使用方法,其中該流感疫苗套組係施用於一個體,並包含以下步驟:a.對該個體施用一第一疫苗組合物;以及b.於施用第一疫苗組合物後約1至10天,對該個體施用一第二疫苗組合物。藉此,對流感病毒的預防將可更有效、更廣泛。