SAMMENSÆTNING TIL SPALTNING AF ET MÅL-DNA OMFATTENDE ET LEDER-RNA SPECIFIKT FOR MÅL-DNA´ET OG CAS PROTEIN-KODENDE NUKLEINSYRE ELLER CAS-PROTEIN, OG ANVENDELSE DERAF
KIM, Jin-Soo,CHO, Seung Woo,KIM, Sojung,KIM, Jong Min,KIM, Seokjoong
申请号：
DK20130849670T
公开号：
DK2912175(T3)
申请日:
2013.10.23
申请国别(地区):
丹麦
年份:
2018
代理人:
摘要：
The invention provides a CRISPR/Cas9 composition for cleaving a target DNA in a plant cell, wherein the target DNA is endogenous DNA, and wherein the composition comprises (a) a nucleic acid encoding a CRISPR-associated protein 9 (Cas9) polypeptide, or a Cas9 polypeptide; and (b) a guide RNA specific to the target DNA, wherein the guide RNA is (i) a dual guide RNA comprising a CRISPR RNA (crRNA) and a trans activating crRNA (tracrRNA); or (ii) a single-chain guide RNA comprising a CRISPR RNA (crRNA) fused to a trans activating crRNA (tracrRNA). The invention further provides a plant cell, in particular a protoplast, and a plant, comprising the composition of the invention as well as a method for cleaving a target DNA or introducing two nicks into a target DNA of a plant cell using the composition of the invention.
