The present invention relates to a novel synergistic herbal formulation for regularization and secretion of insulin. More particularly, the present invention relates to a novel synergistic herbal formulation for regularization and secretion of insulin by using Serpgandha, Kurchi bark, Aswagandha, Jam seed, Bilva Patra, Golmarich, Nayantara Patra, purified Shilajit in particular ratio. Moreover this invention relates to the process for the preparation of the above formulation.