Locksley Earl McGann,Janet Anne Wade Elliott,Lisa Ula Ross-Rodriguez
申请号：
US12656600
公开号：
US09078429B2
申请日:
2010.02.04
申请国别(地区):
US
年份:
2015
代理人:
摘要：
A non-linear cooling cryopreservation method for improving cryopreservation protocols for cells that involves producing a simulation of cellular responses to a range of cooling parameters determining optimal cooling parameters required to minimize cryoinjury to the cells using simulation of cellular responses and experimental results and incorporating optimal parameters into the protocol. The simulation is based on mathematical models of cellular parameters. A non-linear cooling cryopreservation protocol for cryopreserving stem cells is also disclosed that does not require cryoprotectants.
